DGKG polyclonal antibody
产品名称： DGKG polyclonal antibody
英文名称： DGKG polyclonal antibody
产品编号： PAB3342
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic peptide of DGKG.
- Immunogen:
- A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human DGKG.
- Host:
- Rabbit
- Reactivity:
- Human, Mouse
- Form:
- Liquid
- Purification:
- Protein G purification
- Storage Buffer:
- In PBS (0.09% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Recommend Usage:
- ELISA (1:1000)
Western Blot (1:100-500)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- Regulatory role of diacylglycerol kinase gamma in macrophage differentiation of leukemia cells.
Yamada K, Sakane F, Imai S, Tsushima S, Murakami T, Kanoh H.Biochem Biophys Res Commun. 2003 May 23;305(1):101-7.
- 2.
- Mapping and genomic characterization of the gene encoding diacylglycerol kinase gamma (DAGK3): assessment of its role in dominant optic atrophy (OPA1).
Stohr H, Klein J, Gehrig A, Koehler MR, Jurklies B, Kellner U, Leo-Kottler B, Schmid M, Weber BH.Hum Genet. 1999 Jan;104(1):99-105.
- 3.
- Molecular cloning of a diacylglycerol kinase isozyme predominantly expressed in human retina with a truncated and inactive enzyme expression in most other human cells.
Kai M, Sakane F, Imai S, Wada I, Kanoh H.J Biol Chem. 1994 Jul 15;269(28):18492-8.
- Applications
- Western Blot (Tissue lysate)
- The DGKG polyclonal antibody (Cat # PAB3342) is used in Western blot to detect DGKG in mouse small intestine tissue lysate.
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with DGKG polyclonal antibody (Cat # PAB3342) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. HC = hepatocarcinoma.
- Entrez GeneID:
- 1608
- Protein Accession#:
- P49619
- Gene Name:
- DGKG
- Gene Alias:
- DAGK3,DGK-GAMMA,MGC104993,MGC133330
- Gene Description:
- diacylglycerol kinase, gamma 90kDa
- Omim ID:
- 601854
- Gene Ontology:
- Hyperlink
- Gene Summary:
- This gene encodes an enzyme that is a member of the type I subfamily of diacylglycerol kinases, which are involved in lipid metabolism. These enzymes generate phosphatidic acid by catalyzing the phosphorylation of diacylglycerol, a fundamental lipid second messenger that activates numerous proteins, including protein kinase C isoforms, Ras guanyl nucleotide-releasing proteins and some transient receptor potential channels. Diacylglycerol kinase gamma has been implicated in cell cycle regulation and in the negative regulation of macrophage differentiation in leukemia cells. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
- Other Designations:
- DAG kinase gamma,diacylglycerol kinase gamma,diacylglyerol kinase gamma,diglyceride kinase gamma