MYC-tag polyclonal antibody
产品名称： MYC-tag polyclonal antibody
英文名称： MYC-tag polyclonal antibody
产品编号： PAB10345
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic peptide of MYC-tag.
- Immunogen:
- A synthetic peptide (conjugated with KLH) corresponding to amino acids 410-419 of human MYC-tag.
- Sequence:
- EQKLISEEDL
- Host:
- Rabbit
- Reactivity:
- Human
- Specificity:
- This affinity purified antibody is directed against human c-Myc and is useful in determining its presence in various assays. This polyclonal anti-Myc-tag antibody detects overexpressed proteins containing the Myc epitope tag. This antibody recognizes the Myc-tag (Glu-Gln-Lys-Leu-Ile-Ser-Glu-Glu-Asp-Leu) fused to either the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
- Form:
- Liquid
- Storage Buffer:
- In 20 mM potassium phosphate buffer, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Quality Control Testing:
- Antibody Reactive Against Synthetic Peptide.
- Recommend Usage:
- ELISA (1:135000)
Western Blot (1:500-1:5000)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Applications
- Western Blot (Transfected lysate)
- MYC-tag polyclonal antibody (Cat # PAB10345) detects ~60 kDa amino terminal linked MYC-tagged recombinant protein by western blot (arrowhead).
Lane 1 contains ~35 ug of lysate from control 293T cells.
Lane 2 contains ~35 ug of lysate from 293T cells overexpressing an N-terminal linked recombinant protein.
Amino terminal linked MYC recombinant protein was the gift of Brian Conti, University of North Carolina, Chapel Hill, NC.
- Western Blot (Recombinant protein)
- MYC-tag polyclonal antibody (Cat # PAB10345) detects ~ 100 kDa carboxy terminal linked MYC-tagged recombinant protein present in ~35 ug of lysate by western blot (arrowhead).
Carboxy terminal linked MYC recombinant protein was the gift of Zhongsheng You, Salk Institute, LaJolla, CA.
- Entrez GeneID:
- 4609
- Gene Name:
- MYC
- Gene Alias:
- bHLHe39,c-Myc
- Gene Description:
- v-myc myelocytomatosis viral oncogene homolog (avian)
- Gene Ontology:
- Hyperlink
- Gene Summary:
- The protein encoded by this gene is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of hematopoietic tumors, leukemias and lymphomas, including Burkitt lymphoma. There is evidence to show that alternative translation initiations from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site result in the production of two isoforms with distinct N-termini. The synthesis of non-AUG initiated protein is suppressed in Burkitt's lymphomas, suggesting its importance in the normal function of this gene. [provided by RefSeq
- Other Designations:
- avian myelocytomatosis viral oncogene homolog,myc proto-oncogene protein,v-myc avian myelocytomatosis viral oncogene homolog
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