E tag polyclonal antibody
产品名称： E tag polyclonal antibody
英文名称： E tag polyclonal antibody
产品编号： PAB12765
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic peptides of E tag.
- Immunogen:
- A synthetic peptide corresponding to E tag.
- Sequence:
- GAPVPYPDPLEPR
- Host:
- Rabbit
- Specificity:
- This antibody recognizes E-tagged fusion proteins.
- Form:
- Liquid
- Storage Buffer:
- In PBS, pH 7.2 (10% Proclin300)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing.
- Quality Control Testing:
- Antibody Reactive Against Synthetic Peptide.
- Recommend Usage:
- Western Blot (0.1-1 ug/mL)
ELISA (0.01-0.1 ug/mL)
Immunoprecipitation (2-5 ug/mL)
The optimal working dilution should be determined by the end user.
- Publication Reference
- 1.
- The alternative carboxyl termini of avian cardiac and brain sarcoplasmic reticulum/endoplasmic reticulum Ca(2+)-ATPases are on opposite sides of the membrane.
Campbell AM, Kessler PD, Fambrough DM.J Biol Chem. 1992 May 5;267(13):9321-5.
- Applications
- Western Blot (Transfected lysate)
- The whole cell lysate of NIH/ 3T3 with over-expressed E-Tag fused recombinant protein was resolved onto 10% SDS-PAGE, then transferred onto NC membrane. Followed by an immune-blotting with E tag polyclonal antibody (Cat # PAB12765) at 1 : 1000. Lane 2 was pre-incubated by immunizing peptide.